Construct: shRNA TRCN0000215619

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001024926.3-1346s1c1
DNA Barcode:
GGTAATTGACTGAGCTCTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cyb5d2 (192986)
Gene Description:
cytochrome b5 domain containing 2
Transcript:
RefSeq NM_001024926.3 (CURRENT)
Match location:
Position 1346 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000215619)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGTAATTGACTGAGCTCTTAA
Hairpin Sequence:
5'-CCGG-GGTAATTGACTGAGCTCTTAA-CTCGAG-TTAAGAGCTCAGTCAATTACC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGTAATTGACTGAGCTCTTAACTCGAGTTAAGAGCTCAGTCAATTACCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGTAATTGACTGAGCTCTTAACTCGAGTTAAGAGCTCAGTCAATTACC-3'

Other clones with same target sequence:

(none)