Construct: shRNA TRCN0000215698

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001099628.1-7896s1c1
DNA Barcode:
CTTCATCAACTATGGTCTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atad2b (320817)
Gene Description:
ATPase family, AAA domain containing 2B
Transcript:
RefSeq NM_001099628.1 (CURRENT)
Match location:
Position 7896 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000215698)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCATCAACTATGGTCTATT
Hairpin Sequence:
5'-CCGG-CTTCATCAACTATGGTCTATT-CTCGAG-AATAGACCATAGTTGATGAAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCATCAACTATGGTCTATTCTCGAGAATAGACCATAGTTGATGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCATCAACTATGGTCTATTCTCGAGAATAGACCATAGTTGATGAAG-3'

Other clones with same target sequence:

(none)