Construct: shRNA TRCN0000215714

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028372.1-1426s1c1
DNA Barcode:
CTCAACGTAAACTAGCATTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mblac2 (72852)
Gene Description:
metallo-beta-lactamase domain containing 2
Transcript:
RefSeq NM_028372.1 (CURRENT)
Match location:
Position 1426 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000215714)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCAACGTAAACTAGCATTTA
Hairpin Sequence:
5'-CCGG-CTCAACGTAAACTAGCATTTA-CTCGAG-TAAATGCTAGTTTACGTTGAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCAACGTAAACTAGCATTTACTCGAGTAAATGCTAGTTTACGTTGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCAACGTAAACTAGCATTTACTCGAGTAAATGCTAGTTTACGTTGAG-3'

Other clones with same target sequence:

(none)