Construct: shRNA TRCN0000215733

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198161.2-2350s1c1
DNA Barcode:
CAATACTCTTACAGCCATATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bhlhb9 (70237)
Gene Description:
basic helix-loop-helix domain containing, class B9
Transcript:
RefSeq NM_198161.2 (CURRENT)
Match location:
Position 2350 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000215733)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAATACTCTTACAGCCATATT
Hairpin Sequence:
5'-CCGG-CAATACTCTTACAGCCATATT-CTCGAG-AATATGGCTGTAAGAGTATTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAATACTCTTACAGCCATATTCTCGAGAATATGGCTGTAAGAGTATTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAATACTCTTACAGCCATATTCTCGAGAATATGGCTGTAAGAGTATTG-3'

Other clones with same target sequence:

(none)