Construct: shRNA TRCN0000215869

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172698.2-2012s1c1
DNA Barcode:
CTGGAACTAAGATTAGCTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Efcab14 (230648)
Gene Description:
EF-hand calcium binding domain 14
Transcript:
RefSeq NM_172698.2 (CURRENT)
Match location:
Position 2012 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000215869)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGGAACTAAGATTAGCTTTA
Hairpin Sequence:
5'-CCGG-CTGGAACTAAGATTAGCTTTA-CTCGAG-TAAAGCTAATCTTAGTTCCAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGGAACTAAGATTAGCTTTACTCGAGTAAAGCTAATCTTAGTTCCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGGAACTAAGATTAGCTTTACTCGAGTAAAGCTAATCTTAGTTCCAG-3'

Other clones with same target sequence:

  1. TRCN0000266899