Construct: shRNA TRCN0000215966

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175280.3-543s1c1
DNA Barcode:
CATTCCATGTCTGAACTATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cfap61 (78774)
Gene Description:
cilia and flagella associated protein 61
Transcript:
RefSeq NM_175280.3 (CURRENT)
Match location:
Position 543 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000215966)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATTCCATGTCTGAACTATAA
Hairpin Sequence:
5'-CCGG-CATTCCATGTCTGAACTATAA-CTCGAG-TTATAGTTCAGACATGGAATG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATTCCATGTCTGAACTATAACTCGAGTTATAGTTCAGACATGGAATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATTCCATGTCTGAACTATAACTCGAGTTATAGTTCAGACATGGAATG-3'

Other clones with same target sequence:

(none)