Construct: shRNA TRCN0000215977

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024166.6-661s1c1
DNA Barcode:
CAGGATTGCAAATGGTTTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Chchd2 (14004)
Gene Description:
coiled-coil-helix-coiled-coil-helix domain containing 2
Transcript:
RefSeq NM_024166.6 (CURRENT)
Match location:
Position 661 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000215977)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGGATTGCAAATGGTTTAAT
Hairpin Sequence:
5'-CCGG-CAGGATTGCAAATGGTTTAAT-CTCGAG-ATTAAACCATTTGCAATCCTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGGATTGCAAATGGTTTAATCTCGAGATTAAACCATTTGCAATCCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGGATTGCAAATGGTTTAATCTCGAGATTAAACCATTTGCAATCCTG-3'

Other clones with same target sequence:

  1. TRCN0000251042