Construct: shRNA TRCN0000216344

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020278.2-244s1c1
DNA Barcode:
GTAGCAAAGATAACGCTTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lgi1 (56839)
Gene Description:
leucine-rich repeat LGI family, member 1
Transcript:
RefSeq NM_020278.2 (CURRENT)
Match location:
Position 244 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000216344)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTAGCAAAGATAACGCTTTAT
Hairpin Sequence:
5'-CCGG-GTAGCAAAGATAACGCTTTAT-CTCGAG-ATAAAGCGTTATCTTTGCTAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTAGCAAAGATAACGCTTTATCTCGAGATAAAGCGTTATCTTTGCTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTAGCAAAGATAACGCTTTATCTCGAGATAAAGCGTTATCTTTGCTAC-3'

Other clones with same target sequence:

(none)