Construct: shRNA TRCN0000216456

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027000.4-2320s1c1
DNA Barcode:
GAGCTTAAACTACTCTTAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gtpbp4 (69237)
Gene Description:
GTP binding protein 4
Transcript:
RefSeq NM_027000.4 (CURRENT)
Match location:
Position 2320 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000216456)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGCTTAAACTACTCTTAAAT
Hairpin Sequence:
5'-CCGG-GAGCTTAAACTACTCTTAAAT-CTCGAG-ATTTAAGAGTAGTTTAAGCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGCTTAAACTACTCTTAAATCTCGAGATTTAAGAGTAGTTTAAGCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGCTTAAACTACTCTTAAATCTCGAGATTTAAGAGTAGTTTAAGCTC-3'

Other clones with same target sequence:

(none)