Construct: shRNA TRCN0000216539

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198668.2-3104s1c1
DNA Barcode:
GATGATACTGAGTCAACTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
4930590J08Rik (381798)
Gene Description:
RIKEN cDNA 4930590J08 gene
Transcript:
RefSeq NM_198668.2 (CURRENT)
Match location:
Position 3104 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000216539)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGATACTGAGTCAACTAAA
Hairpin Sequence:
5'-CCGG-GATGATACTGAGTCAACTAAA-CTCGAG-TTTAGTTGACTCAGTATCATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGATACTGAGTCAACTAAACTCGAGTTTAGTTGACTCAGTATCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGATACTGAGTCAACTAAACTCGAGTTTAGTTGACTCAGTATCATC-3'

Other clones with same target sequence:

  1. TRCN0000265130