Construct: shRNA TRCN0000216645
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_133829.2-2453s1c1
- DNA Barcode:
- CTATGCTCTCTACCAAGTTAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Mfsd6 (98682)
- Gene Description:
- major facilitator superfamily domain containing 6
- Transcript:
- RefSeq NM_133829.2 (CURRENT)
- Match location:
- Position 2453 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000216645)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CTATGCTCTCTACCAAGTTAA
- Hairpin Sequence:
-
5'-CCGG-CTATGCTCTCTACCAAGTTAA-CTCGAG-TTAACTTGGTAGAGAGCATAG-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCTATGCTCTCTACCAAGTTAACTCGAGTTAACTTGGTAGAGAGCATAGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACTATGCTCTCTACCAAGTTAACTCGAGTTAACTTGGTAGAGAGCATAG-3'
Other clones with same target sequence:
-
TRCN0000248378
