Construct: shRNA TRCN0000216854

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178614.4-1012s1c1
DNA Barcode:
GCTTCATCAAGGAAGACTTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Samm50 (68653)
Gene Description:
SAMM50 sorting and assembly machinery component
Transcript:
RefSeq NM_178614.4 (CURRENT)
Match location:
Position 1012 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000216854)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTCATCAAGGAAGACTTTG
Hairpin Sequence:
5'-CCGG-GCTTCATCAAGGAAGACTTTG-CTCGAG-CAAAGTCTTCCTTGATGAAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTCATCAAGGAAGACTTTGCTCGAGCAAAGTCTTCCTTGATGAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTCATCAAGGAAGACTTTGCTCGAGCAAAGTCTTCCTTGATGAAGC-3'

Other clones with same target sequence:

(none)