Construct: shRNA TRCN0000216960

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177381.3-2009s1c1
DNA Barcode:
CCTATGACTGTTCCACGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cog3 (338337)
Gene Description:
component of oligomeric golgi complex 3
Transcript:
RefSeq NM_177381.3 (CURRENT)
Match location:
Position 2009 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000216960)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTATGACTGTTCCACGATTT
Hairpin Sequence:
5'-CCGG-CCTATGACTGTTCCACGATTT-CTCGAG-AAATCGTGGAACAGTCATAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTATGACTGTTCCACGATTTCTCGAGAAATCGTGGAACAGTCATAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTATGACTGTTCCACGATTTCTCGAGAAATCGTGGAACAGTCATAGG-3'

Other clones with same target sequence:

  1. TRCN0000248282