Construct: shRNA TRCN0000217187

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027357.2-1566s1c1
DNA Barcode:
CAAGAATGCCAGCAATGATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Psmd1 (70247)
Gene Description:
proteasome (prosome, macropain) 26S subunit, non-ATPase, 1
Transcript:
RefSeq NM_027357.2 (CURRENT)
Match location:
Position 1566 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000217187)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGAATGCCAGCAATGATAT
Hairpin Sequence:
5'-CCGG-CAAGAATGCCAGCAATGATAT-CTCGAG-ATATCATTGCTGGCATTCTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGAATGCCAGCAATGATATCTCGAGATATCATTGCTGGCATTCTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGAATGCCAGCAATGATATCTCGAGATATCATTGCTGGCATTCTTG-3'

Other clones with same target sequence:

(none)