Construct: shRNA TRCN0000217290

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025394.3-689s1c1
DNA Barcode:
GTGCTTGAGTACTGCCTTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tomm7 (66169)
Gene Description:
translocase of outer mitochondrial membrane 7 homolog (yeast)
Transcript:
RefSeq NM_025394.3 (CURRENT)
Match location:
Position 689 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000217290)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGCTTGAGTACTGCCTTAAA
Hairpin Sequence:
5'-CCGG-GTGCTTGAGTACTGCCTTAAA-CTCGAG-TTTAAGGCAGTACTCAAGCAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGCTTGAGTACTGCCTTAAACTCGAGTTTAAGGCAGTACTCAAGCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGCTTGAGTACTGCCTTAAACTCGAGTTTAAGGCAGTACTCAAGCAC-3'

Other clones with same target sequence:

(none)