Construct: shRNA TRCN0000217583

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026516.2-1199s1c1
DNA Barcode:
GAATTGCACTGCAGGACATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tmem178 (68027)
Gene Description:
transmembrane protein 178
Transcript:
RefSeq NM_026516.2 (CURRENT)
Match location:
Position 1199 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000217583)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAATTGCACTGCAGGACATTT
Hairpin Sequence:
5'-CCGG-GAATTGCACTGCAGGACATTT-CTCGAG-AAATGTCCTGCAGTGCAATTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAATTGCACTGCAGGACATTTCTCGAGAAATGTCCTGCAGTGCAATTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAATTGCACTGCAGGACATTTCTCGAGAAATGTCCTGCAGTGCAATTC-3'

Other clones with same target sequence:

(none)