Construct: shRNA TRCN0000217640

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026149.4-1525s1c1
DNA Barcode:
GGCAGCACATGGACAATTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nudcd1 (67429)
Gene Description:
NudC domain containing 1
Transcript:
RefSeq NM_026149.4 (CURRENT)
Match location:
Position 1525 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000217640)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGCAGCACATGGACAATTAAA
Hairpin Sequence:
5'-CCGG-GGCAGCACATGGACAATTAAA-CTCGAG-TTTAATTGTCCATGTGCTGCC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGCAGCACATGGACAATTAAACTCGAGTTTAATTGTCCATGTGCTGCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGCAGCACATGGACAATTAAACTCGAGTTTAATTGTCCATGTGCTGCC-3'

Other clones with same target sequence:

(none)