Construct: shRNA TRCN0000217714

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178939.2-301s1c1
DNA Barcode:
GATCAAGAGCACCTGGATAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pdrg1 (68559)
Gene Description:
p53 and DNA damage regulated 1
Transcript:
RefSeq NM_178939.2 (CURRENT)
Match location:
Position 301 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000217714)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCAAGAGCACCTGGATAAA
Hairpin Sequence:
5'-CCGG-GATCAAGAGCACCTGGATAAA-CTCGAG-TTTATCCAGGTGCTCTTGATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCAAGAGCACCTGGATAAACTCGAGTTTATCCAGGTGCTCTTGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCAAGAGCACCTGGATAAACTCGAGTTTATCCAGGTGCTCTTGATC-3'

Other clones with same target sequence:

(none)