Construct: shRNA TRCN0000217727

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027113.2-1372s1c1
DNA Barcode:
GGATTCTGGCAAAGCTTAAAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wdr5b (69544)
Gene Description:
WD repeat domain 5B
Transcript:
RefSeq NM_027113.2 (CURRENT)
Match location:
Position 1372 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000217727)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGATTCTGGCAAAGCTTAAAC
Hairpin Sequence:
5'-CCGG-GGATTCTGGCAAAGCTTAAAC-CTCGAG-GTTTAAGCTTTGCCAGAATCC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGATTCTGGCAAAGCTTAAACCTCGAGGTTTAAGCTTTGCCAGAATCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGATTCTGGCAAAGCTTAAACCTCGAGGTTTAAGCTTTGCCAGAATCC-3'

Other clones with same target sequence:

(none)