Construct: shRNA TRCN0000217839

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_134003.1-353s1c1
DNA Barcode:
CATGAGTGAGGTATCCAATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zc3h10 (103284)
Gene Description:
zinc finger CCCH type containing 10
Transcript:
RefSeq NM_134003.1 (CURRENT)
Match location:
Position 353 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000217839)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATGAGTGAGGTATCCAATTT
Hairpin Sequence:
5'-CCGG-CATGAGTGAGGTATCCAATTT-CTCGAG-AAATTGGATACCTCACTCATG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATGAGTGAGGTATCCAATTTCTCGAGAAATTGGATACCTCACTCATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATGAGTGAGGTATCCAATTTCTCGAGAAATTGGATACCTCACTCATG-3'

Other clones with same target sequence:

  1. TRCN0000241950