Construct: shRNA TRCN0000217896

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178795.4-1566s1c1
DNA Barcode:
GGTCACTTCTCAGGTATTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ppip5k1 (327655)
Gene Description:
diphosphoinositol pentakisphosphate kinase 1
Transcript:
RefSeq NM_178795.4 (CURRENT)
Match location:
Position 1566 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000217896)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGTCACTTCTCAGGTATTAAT
Hairpin Sequence:
5'-CCGG-GGTCACTTCTCAGGTATTAAT-CTCGAG-ATTAATACCTGAGAAGTGACC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGTCACTTCTCAGGTATTAATCTCGAGATTAATACCTGAGAAGTGACCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGTCACTTCTCAGGTATTAATCTCGAGATTAATACCTGAGAAGTGACC-3'

Other clones with same target sequence:

(none)