Construct: shRNA TRCN0000217944

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178644.3-1031s1c1
DNA Barcode:
GCTACACCTTTGACTTCTATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Oaf (102644)
Gene Description:
out at first homolog
Transcript:
RefSeq NM_178644.3 (CURRENT)
Match location:
Position 1031 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000217944)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTACACCTTTGACTTCTATG
Hairpin Sequence:
5'-CCGG-GCTACACCTTTGACTTCTATG-CTCGAG-CATAGAAGTCAAAGGTGTAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTACACCTTTGACTTCTATGCTCGAGCATAGAAGTCAAAGGTGTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTACACCTTTGACTTCTATGCTCGAGCATAGAAGTCAAAGGTGTAGC-3'

Other clones with same target sequence:

(none)