Construct: shRNA TRCN0000219478

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178892.5-1901s1c1
DNA Barcode:
GTTTGGACAAGGCAGTTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tiparp (99929)
Gene Description:
TCDD-inducible poly(ADP-ribose) polymerase
Transcript:
RefSeq NM_178892.5 (CURRENT)
Match location:
Position 1901 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000219478)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTTGGACAAGGCAGTTATTT
Hairpin Sequence:
5'-CCGG-GTTTGGACAAGGCAGTTATTT-CTCGAG-AAATAACTGCCTTGTCCAAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTTGGACAAGGCAGTTATTTCTCGAGAAATAACTGCCTTGTCCAAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTTGGACAAGGCAGTTATTTCTCGAGAAATAACTGCCTTGTCCAAAC-3'

Other clones with same target sequence:

  1. TRCN0000273168