Construct: shRNA TRCN0000219595

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033569.3-678s1c1
DNA Barcode:
ATCATCGAGATCGAGATTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cnnm2 (94219)
Gene Description:
cyclin M2
Transcript:
RefSeq NM_033569.3 (CURRENT)
Match location:
Position 678 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000219595)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCATCGAGATCGAGATTAAA
Hairpin Sequence:
5'-CCGG-ATCATCGAGATCGAGATTAAA-CTCGAG-TTTAATCTCGATCTCGATGAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCATCGAGATCGAGATTAAACTCGAGTTTAATCTCGATCTCGATGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCATCGAGATCGAGATTAAACTCGAGTTTAATCTCGATCTCGATGAT-3'

Other clones with same target sequence:

(none)