Construct: shRNA TRCN0000220736

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010790.2-1606s1c2, TRCN0000024065, NM_010790.2-1606s1c1
DNA Barcode:
CGGTTAGAACACCAGGGAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Melk (17279)
Gene Description:
maternal embryonic leucine zipper kinase
Transcript:
RefSeq NM_010790.2 (CURRENT)
Match location:
Position 1606 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220736)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTTAGAACACCAGGGAATT
Hairpin Sequence:
5'-CCGG-CGGTTAGAACACCAGGGAATT-CTCGAG-AATTCCCTGGTGTTCTAACCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTTAGAACACCAGGGAATTCTCGAGAATTCCCTGGTGTTCTAACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTTAGAACACCAGGGAATTCTCGAGAATTCCCTGGTGTTCTAACCG-3'

Other clones with same target sequence:

(none)