Construct: shRNA TRCN0000220999

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146474.1-594s1c2, TRCN0000030189, NM_146474.1-594s1c1
DNA Barcode:
CGGGTCACTGTGTTTACTCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Olfr1261 (258466)
Gene Description:
olfactory receptor 1261
Transcript:
RefSeq NM_146474.1 (CURRENT)
Match location:
Position 594 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000220999)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGTCACTGTGTTTACTCAT
Hairpin Sequence:
5'-CCGG-CGGGTCACTGTGTTTACTCAT-CTCGAG-ATGAGTAAACACAGTGACCCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGTCACTGTGTTTACTCATCTCGAGATGAGTAAACACAGTGACCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGTCACTGTGTTTACTCATCTCGAGATGAGTAAACACAGTGACCCG-3'

Other clones with same target sequence:

(none)