Construct: shRNA TRCN0000221430
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_026512.1-555s1c2, TRCN0000032897, NM_026512.1-555s1c1
- DNA Barcode:
- GTATCCGAGATGTTTCTAAAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Bphl (68021)
- Gene Description:
- biphenyl hydrolase-like (serine hydrolase, breast epithelial mucin-associated antigen)
- Transcript:
- RefSeq NM_026512.1 (CURRENT)
- Match location:
- Position 555 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000221430)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GTATCCGAGATGTTTCTAAAT
- Hairpin Sequence:
-
5'-CCGG-GTATCCGAGATGTTTCTAAAT-CTCGAG-ATTTAGAAACATCTCGGATAC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGTATCCGAGATGTTTCTAAATCTCGAGATTTAGAAACATCTCGGATACTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGTATCCGAGATGTTTCTAAATCTCGAGATTTAGAAACATCTCGGATAC-3'
Other clones with same target sequence:
-
TRCN0000317998
