Construct: shRNA TRCN0000221811

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011196.1-448s1c2, TRCN0000026637, NM_011196.1-448s1c1
DNA Barcode:
CGGGCTAACCATGACAGTGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ptger3 (19218)
Gene Description:
prostaglandin E receptor 3 (subtype EP3)
Transcript:
RefSeq NM_011196.1 (NON-CURRENT)
Match location:
Position 448 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000221811)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGCTAACCATGACAGTGTT
Hairpin Sequence:
5'-CCGG-CGGGCTAACCATGACAGTGTT-CTCGAG-AACACTGTCATGGTTAGCCCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGCTAACCATGACAGTGTTCTCGAGAACACTGTCATGGTTAGCCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGCTAACCATGACAGTGTTCTCGAGAACACTGTCATGGTTAGCCCG-3'

Other clones with same target sequence:

(none)