Construct: shRNA TRCN0000287918

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008139.5-896s21c1
DNA Barcode:
GCTTGTGGAATGATCCTGGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gnaq (14682)
Gene Description:
guanine nucleotide binding protein, alpha q polypeptide
Transcript:
RefSeq NM_008139.5 (CURRENT)
Match location:
Position 896 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000287918)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTGTGGAATGATCCTGGAA
Hairpin Sequence:
5'-CCGG-GCTTGTGGAATGATCCTGGAA-CTCGAG-TTCCAGGATCATTCCACAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTGTGGAATGATCCTGGAACTCGAGTTCCAGGATCATTCCACAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTGTGGAATGATCCTGGAACTCGAGTTCCAGGATCATTCCACAAGC-3'

Other clones with same target sequence:

  1. TRCN0000097888