Construct: shRNA TRCN0000427693

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001082575.1-547s21c1
DNA Barcode:
GCGGCAAATGTTCGGGCAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RBFOX3 (146713)
Gene Description:
RNA binding fox-1 homolog 3
Transcript:
RefSeq NM_001082575.1 (NON-CURRENT)
Match location:
Position 547 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000427693)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGCAAATGTTCGGGCAATT
Hairpin Sequence:
5'-CCGG-GCGGCAAATGTTCGGGCAATT-CTCGAG-AATTGCCCGAACATTTGCCGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGCAAATGTTCGGGCAATTCTCGAGAATTGCCCGAACATTTGCCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGCAAATGTTCGGGCAATTCTCGAGAATTGCCCGAACATTTGCCGC-3'

Other clones with same target sequence:

  1. TRCN0000254935