Construct: shRNA TRCN0000432971

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018922.2-194s21c1
DNA Barcode:
GGGTTAGTGCAGAGGATTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PCDHGB1 (56104)
Gene Description:
protocadherin gamma subfamily B, 1
Transcript:
RefSeq NM_018922.2 (CURRENT)
Match location:
Position 194 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000432971)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGGTTAGTGCAGAGGATTATT
Hairpin Sequence:
5'-CCGG-GGGTTAGTGCAGAGGATTATT-CTCGAG-AATAATCCTCTGCACTAACCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGGTTAGTGCAGAGGATTATTCTCGAGAATAATCCTCTGCACTAACCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGGTTAGTGCAGAGGATTATTCTCGAGAATAATCCTCTGCACTAACCC-3'

Other clones with same target sequence:

(none)