Construct: ORF TRCN0000465211
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008820.1_s317c1
- Derived from:
- ccsbBroadEn_03541
- DNA Barcode:
- CTCCTTCAACCCACCTAAAAGTCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRPS18A (55168)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465211
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55168 | MRPS18A | mitochondrial ribosomal pro... | NM_018135.3 | 100% | 100% | |
| 2 | human | 55168 | MRPS18A | mitochondrial ribosomal pro... | XM_006715134.3 | 72.5% | 72.5% | 374_595del |
| 3 | human | 55168 | MRPS18A | mitochondrial ribosomal pro... | NM_001193343.1 | 64.7% | 64.2% | 377_378insTCTAT;381_382ins202 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 654
- ORF length:
- 588
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggccctcaag gctctggtgt ccggctgtgg gcggcttctc cgtgggctac 121 tagcgggccc ggcagcgacc agctggtctc ggcttccagc tcgcgggttc agggaagtgg 181 tggagaccca agaagggaag acaactataa ttgaaggccg tatcacagcg actcccaagg 241 agagtccaaa tcctcctaac ccctctggcc agtgccccat ctgccgttgg aacctgaagc 301 acaagtataa ctatgacgat gttctgctgc ttagccagtt catccggcct catgGAGGCA 361 TGCTGCCCCG AAAGATCACA GGCCTATGCC AGGAAGAACA CCGCAAGATC GAGGAGTGTG 421 TGAAGATGGC CCACCGAGCA GGTCTATTAC CAAATCACAG GCCTCGGCTT CCTGAAGGAG 481 TTGTTCCGAA GAGCAAACCC CAACTCAACC GGTACCTGAC GCGCTGGGCT CCTGGCTCCG 541 TCAAGCCCAT CTACAAAAAA GGCCCCCGCT GGAACAGGGT GCGCATGCCC GTGGGGTCAC 601 CCCTTCTGAG GGACAATGTC TGCTACTCAA GAACACCTTG GAAGCTGTAT CACTGCCCAA 661 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 721 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 781 TATCTTGTGG AAAGGACGAC TCCTTCAACC CACCTAAAAG TCGACGCGTT AAGTCgacaa 841 tcaacctctg gattacaaaa tttgtgaaag att