Construct: ORF TRCN0000465215
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000807.1_s317c1
- Derived from:
- ccsbBroadEn_16070
- DNA Barcode:
- TTCATTACCATAACTCGGTGGCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCDC114 (93233)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465215
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 93233 | CCDC114 | coiled-coil domain containi... | NM_144577.4 | 69% | 69.1% | 1_621del;807C>T;1908T>C |
| 2 | human | 93233 | CCDC114 | coiled-coil domain containi... | NM_001364171.2 | 65.3% | 65.4% | 1_732del;918C>T;2019T>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1458
- ORF length:
- 1389
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaggcgcag gtcctgcagc ggcagatctt gcacctggag cagctgcacc 121 acttcctcaa gctcaagaac aacgaccggc agccggatcc cgatgtcctg gagaagcgtg 181 aaaagcaggc cggggaggtg gccgagggcg tctggaagac ctcccaggag aggctggtgc 241 tttgctacga ggatgccctg aataaactgt cccagctgat gggggagagt gaccctgacc 301 tgttggtgca gaagtatctg gagatcgagg agcgcaactt tgctgagttc aacttcatca 361 acgagcagaa cttggagctg gagcatgtgc aggaagagat caaggagatg caggaggctt 421 tggtgagcgc acgtgccagc aaggatgacc agcatttgct gcaggagcag cagcagaagg 481 tgttgcagca gcgcatggac aaggtgcact cggaggctga gcgccttgag gcccgcttcc 541 aggatgtgcg gggacagctg gagaagctca aggctgatat ccagctcctc ttcaccaagg 601 cccattgcga cagcagcatg atcgatgacc tccttggggt caagaccagc atgggagacc 661 gggacatggg cctcttcctg agcctcattg agaagcggct ggtggagctc ctgacagtgc 721 aggccttcct acatgcccag agcttcacct ccctggccga cgctgccctc ctagtgctgg 781 gccagagcct ggaggacctt ccgaagaaga tggccccact tcagccccct gacactctag 841 aagacccccc gggttttgag gccagcgatg actaccccat gagcagggag gagctgctga 901 gccaagtgga gaagctggtg gagctccagg agcaggcgga ggcgcagcgc cagaaggacc 961 tggccgccgc cgccgcgaag ctggacggca ccctgagcgt ggacctggcc agcacccaga 1021 gggccggctc cagtaccgtc ctggtgccca ccaggcaccc ccatgccatc cccgggtcca 1081 ttttgagcca caagactagc agagaccgtg gctctcttgg ccacgtcact tttggcggcc 1141 tcagctccag cactgggcat ttgcccagcc acatcacgca cggtgacccc aacactggcc 1201 acgtgacctt cggctccacc agtgcctcga gtgggggcca cgtgaccttc agacccgtca 1261 gcgccagcag ctacctgggc tccactGGAT ACGTGGGGTC CAGCAGGGGC GGAGAAAACA 1321 CAGAGGGTGG TGTGGAGAGC GGAGGCACAG CGTCCGATTC GAGCGGAGGC CTCGGGTCCA 1381 GCAGAGACCA CGTCTCCAGC ACCGGCCCTG CCTCCAGCAC TGGCCCGGGC TCCTCCACCA 1441 GCAAAGACTC CCGGGGCTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1501 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1561 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATTCATTA CCATAACTCG 1621 GTGGCCAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt