Construct: ORF TRCN0000465234
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010265.1_s317c1
- Derived from:
- ccsbBroadEn_02257
- DNA Barcode:
- CATACCCCGGGCCCCCAGCACACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZBTB24 (9841)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465234
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9841 | ZBTB24 | zinc finger and BTB domain ... | NM_001164313.1 | 100% | 100% | |
| 2 | human | 9841 | ZBTB24 | zinc finger and BTB domain ... | NM_014797.2 | 46.7% | 45.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1065
- ORF length:
- 999
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agaaacatcg ccagagcctt ctgggcagct tgttgtacac tcagacgctc 121 acagtgacac tgtgctggcc agttttgagg atcagaggaa gaaaggcttc ctctgtgaca 181 ttactttaat cgtggagaat gtacatttcc gggcccacaa agccttactt gctgccagta 241 gtgaatactt ctcaatgatg tttgcagaag agggggaaat cggccaatcc atttatatgc 301 tggaaggcat ggttgcagac acctttggta tcctgctgga atttatctac acaggttatc 361 tccatgccag tgagaaaagt acagaacaaa tcctggctac tgctcagttc ttaaaagtct 421 atgacctggt aaaggcttac acagacttcc aaaataatca tagctcccca aagccaacaa 481 ctttgaacac tgctggtgcc ccagtggttg ttatctctaa taagaaaaac gatcctccaa 541 agcggaaacg gggaagacca aaaaaagtca atacattgca ggaggagaaa tcagaactgg 601 ctgcagagga agaaatacag ttaagagtga acaattcagt tcagaataga caaaactttg 661 tggttaaagg agacagtggt gtactgaatg agcaaattgc agcaaaagaa aaggaagaat 721 cggagcctac ttgtgagcca agtagagagg aggaaatgcc agttgaaaaa gatgagaact 781 atgatCCCAA GACCGAGGAT GGCCAGGCAA GCCAGAGTCG ATACAGCAAG CGGAGGATTT 841 GGAGATCCGT CAAACTTAAA GATTACAAAC TTGTTGGGGA TCAAGAGGAC CATGGTTCAG 901 CCAAGAGGAT CTGTGGAAGG AGAAAGCGCC CTGGAGGCCC TGAGGCCCGC TGTAAAGACT 961 GTGGCAAGGT CTTTAAGTAC AATCACTTTT TAGCAATCCA CCAGAGGAGC CACACAGGTA 1021 ATGATGTTTT CAAAGCTGAT TGCAGTGTGT TGCAGAATTG GGAGTACCCA ACTTTCTTGT 1081 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1141 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1201 GAAAGGACGA CATACCCCGG GCCCCCAGCA CACCACGCGT TAAGTCgaca atcaacctct 1261 ggattacaaa atttgtgaaa gatt