Construct: ORF TRCN0000465247
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012197.1_s317c1
- Derived from:
- ccsbBroadEn_05761
- DNA Barcode:
- AGGCCGCTTCAGATCCCCCCAATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACP3 (55)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465247
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55 | ACP3 | acid phosphatase 3 | NM_001099.5 | 99.3% | 98.9% | (many diffs) |
| 2 | human | 55 | ACP3 | acid phosphatase 3 | XM_011512946.1 | 98.1% | 96.9% | (many diffs) |
| 3 | human | 55 | ACP3 | acid phosphatase 3 | NM_001134194.2 | 90.9% | 89.9% | (many diffs) |
| 4 | human | 55 | ACP3 | acid phosphatase 3 | NM_001292037.2 | 90.8% | 90.4% | (many diffs) |
| 5 | human | 55 | ACP3 | acid phosphatase 3 | XM_011512947.2 | 83% | 82% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1224
- ORF length:
- 1158
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag agctgcaccc ctcctcctgg ccagggcagc aagccttaac cttggcttct 121 tgtttctgct ttttttctgg ctagaccgaa gtgtactagc caaggagttg aagtttgtga 181 ctttggtgtt tcggcatgga gaccgaagtc ccattgacac ctttcccact gaccccataa 241 aggaatcctc atggccacaa ggatttggcc aactcaccca gctgggcatg gagcagcatt 301 atgaacttgg agagtatata agaaagagat atagaaaatt cttgaatgag tcctataaac 361 atgaacaggt ttatattcga agcacagacg ttgaccggac tttgatgagt gctatgacaa 421 acttggcagc cctggttccc ccagaaggtg tcagcatctg gaatcctatc ctactctggc 481 agcccatccc ggtgcacaca gttcctcttt ctgaagatca gttgctatac ctgcctttca 541 ggaactgccc tcgttttcaa gaacttgaga gtgagacttt gaaatcagag gaattccaga 601 agaggctgca cccttataag gattttatag ctaccttggg aaaactttca ggattacatg 661 gccaggacct ttttggaatt tggagtaaag tctacgaccc tttatattgt gagagtgttc 721 acaatttcac tttaccctcc cgggccactg aggacaccat gactaagttg agagaattgt 781 cagaattgtc cctcctgtcc ctctatggaa ttcacaagca gaaagagaaa tctaggctcc 841 aagggggtgt cctggtcaat gaaatcctca atcacatgaa gagagcaact cagataccaa 901 gctacaaaaa actcatcatg tattctgcgc atgacactac tgtgagtggc ctacagatgg 961 cgctagatgt ttacaacgga ctccttcctc cctatgcttc ttgccacttg acggaattgt 1021 actttgagaa gggggagtac tttgtggaga tgtactatcg gaatgagacg cagcacgagc 1081 cgtatcccct catgctacct ggctgcagcc ccagctgtcc tctggagagg tttgctgagc 1141 tggttggccc tgtgatccct caagactggt ccacggagtg tatgaccaca aacagccatc 1201 aaggtactga agacagtaca TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1261 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1321 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAAGGC CGCTTCAGAT 1381 CCCCCCAATG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt