Construct: ORF TRCN0000465251
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018479.1_s317c1
- Derived from:
- ccsbBroadEn_10501
- DNA Barcode:
- ACATGAAGGACGGCGCTCCTATCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GSTT4 (25774)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465251
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25774 | GSTT4 | glutathione S-transferase t... | XM_024452202.1 | 39.4% | 39.5% | (many diffs) |
| 2 | human | 25774 | GSTT4 | glutathione S-transferase t... | XM_024452199.1 | 29.7% | 29.7% | (many diffs) |
| 3 | human | 25774 | GSTT4 | glutathione S-transferase t... | NM_001358664.2 | 27.6% | 27.3% | (many diffs) |
| 4 | human | 25774 | GSTT4 | glutathione S-transferase t... | NR_003081.3 | 24.8% | (many diffs) | |
| 5 | human | 653399 | GSTTP2 | glutathione S-transferase t... | NR_003082.1 | 8.4% | (many diffs) | |
| 6 | mouse | 75886 | Gstt4 | glutathione S-transferase, ... | NM_029472.3 | 23.1% | 24.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 273
- ORF length:
- 204
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaAAAG 61 TTGGCACCAT GGCCCTGGAG CTCTACATGG ACCTGCTGTC AGCACCCTGC CGTGCCGTCT 121 ACATCTTCTC GAAGAAGCAT GACATCCAGT TCAACTTTCA GTTTGTGGAT CTGCTGAAAG 181 GTCACCACCA CAGCAAAGAA TACATTGACA TCAACCCCCT CAGGAAGCTG CCCAGCCTCA 241 AAGATGGGAA ATTTATCTTA AGTGAAAGTT GCTTGCCAAC TTTCTTGTAC AAAGTGGTTG 301 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 361 GCCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAC 421 ATGAAGGACG GCGCTCCTAT CGACGCGTTA AGTCgacaat caacctctgg attacaaaat 481 ttgtgaaaga tt