Construct: ORF TRCN0000465255
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000299.1_s317c1
- Derived from:
- ccsbBroadEn_06275
- DNA Barcode:
- GTCACGATCCTCAATATGTACTGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GJA3 (2700)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465255
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2700 | GJA3 | gap junction protein alpha 3 | NM_021954.4 | 99.8% | 99.7% | 895C>A;1017G>A |
| 2 | human | 2700 | GJA3 | gap junction protein alpha 3 | XM_011535048.2 | 99.8% | 99.7% | 895C>A;1017G>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1374
- ORF length:
- 1305
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggcgactgg agctttctgg gaagactctt agaaaatgca caggagcact 121 ccacggtcat cggcaaggtt tggctgaccg tgctgttcat cttccgcatc ttggtgctgg 181 gggccgcggc ggaggacgtg tggggcgatg agcagtcaga cttcacctgc aacacccagc 241 agccgggctg cgagaacgtc tgctacgaca gggccttccc catctcccac atccgcttct 301 gggcgctgca gatcatcttc gtgtccacgc ccaccctcat ctacctgggc cacgtgctgc 361 acatcgtgcg catggaagag aagaagaaag agagggagga ggaggagcag ctgaagagag 421 agagccccag ccccaaggag ccaccgcagg acaatccctc gtcgcgggac gaccgcggca 481 gggtgcgcat ggccggggcg ctgctgcgga cctacgtctt caacatcatc ttcaagacgc 541 tgttcgaggt gggcttcatc gccggccagt actttctgta cggcttcgag ctgaagccgc 601 tctaccgctg cgaccgctgg ccctgcccca acacggtgga ctgcttcatc tccaggccca 661 cggagaagac catcttcatc atcttcatgc tggcggtggc ctgcgcgtcc ctgctgctca 721 acatgctgga gatctaccac ctgggctgga agaagctcaa gcagggcgtg accagccgcc 781 tcggcccgga cgcctccgag gccccgctgg ggacagccga tcccccgccc ctgcccccca 841 gctcccggcc gcccgccgtt gccatcgggt tcccacccta ctatgcgcac accgctgcgc 901 ccctgggaca ggcccgcgcc gtgggctacc ccggggcccc gccaccagcc gcggacttca 961 aaatgctagc cctgaccgag gcgcgcggaa agggccagtc cgccaagctc tacaacggcc 1021 accaccacct gctgatgact gagcagaact gggCCAACCA GGCGGCCGAG CGGCAGCCCC 1081 CGGCACTCAA GGCTTACCCG GCAGCGTCCA CGCCTGCAGC CCCCAGCCCC GTCGGCAGCA 1141 GCTCCCCGCC ACTCGCGCAC GAGGCTGAGG CGGGCGCGGC GCCCCTGCTG CTGGATGGGA 1201 GCGGCAGCAG TCTGGAGGGG AGCGCCCTGG CAGGGACCCC CGAGGAGGAG GAGCAGGCCG 1261 TGACCACCGC GGCCCAGATG CACCAGCCGC CCTTGCCCCT CGGAGACCCA GGTCGGGCCA 1321 GCAAGGCCAG CAGGGCCAGC AGCGGGCGGG CCAGACCGGA GGACTTGGCC ATCTTGCCAA 1381 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1441 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1501 TATCTTGTGG AAAGGACGAG TCACGATCCT CAATATGTAC TGGACGCGTT AAGTCgacaa 1561 tcaacctctg gattacaaaa tttgtgaaag att