Construct: ORF TRCN0000465256
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014065.1_s317c1
- Derived from:
- ccsbBroadEn_01031
- DNA Barcode:
- TGGGTTGCTTGTCCAAACATGATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MPG (4350)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465256
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4350 | MPG | N-methylpurine DNA glycosylase | NM_001015052.3 | 100% | 100% | |
| 2 | human | 4350 | MPG | N-methylpurine DNA glycosylase | NM_002434.4 | 97.7% | 95.9% | (many diffs) |
| 3 | human | 4350 | MPG | N-methylpurine DNA glycosylase | XM_024450282.1 | 97.7% | 95.9% | (many diffs) |
| 4 | human | 4350 | MPG | N-methylpurine DNA glycosylase | NM_001015054.2 | 95.9% | 95.9% | 0_1ins36 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 945
- ORF length:
- 879
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc cgcgcgcagc ggggcccagt tttgccgacg gatggggcaa aagaagcagc 121 gaccagctag agcagggcag ccacacagct cgtccgacgc agcccaggca cctgcagagc 181 agccacacag ctcgtccgat gcagcccagg caccttgccc cagggagcgc tgcttgggac 241 cgcccaccac tccgggccca taccgcagca tctatttctc aagcccaaag ggccacctta 301 cccgactggg gttggagttc ttcgaccagc cggcagtccc cctggcccgg gcatttctgg 361 gacaggtcct agtccggcga cttcctaatg gcacagaact ccgaggccgc atcgtggaga 421 ccgaggcata cctggggcca gaggatgaag ccgcccactc aaggggtggc cggcagaccc 481 cccgcaaccg aggcatgttc atgaagccgg ggaccctgta cgtgtacatc atttacggca 541 tgtacttctg catgaacatc tccagccagg gggacggggc ttgcgtcttg ctgcgagcac 601 tggagcccct ggaaggtctg gagaccatgc gtcagcttcg cagcacccTC CGGAAAGGCA 661 CCGCCAGCCG TGTCCTCAAG GACCGCGAGC TCTGCAGTGG CCCCTCCAAG CTGTGCCAGG 721 CCCTGGCCAT CAACAAGAGC TTTGACCAGA GGGACCTGGC ACAGGATGAA GCTGTATGGC 781 TGGAGCGTGG TCCCCTGGAG CCCAGTGAGC CGGCTGTAGT GGCAGCAGCC CGGGTGGGCG 841 TCGGCCATGC AGGGGAGTGG GCCCGGAAAC CCCTCCGCTT CTATGTCCGG GGCAGCCCCT 901 GGGTCAGTGT GGTCGACAGA GTGGCTGAGC AGGACACACA GGCCTGCCCA ACTTTCTTGT 961 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1021 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1081 GAAAGGACGA TGGGTTGCTT GTCCAAACAT GATTACGCGT TAAGTCgaca atcaacctct 1141 ggattacaaa atttgtgaaa gatt