Construct: ORF TRCN0000465268
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016328.1_s317c1
- Derived from:
- ccsbBroadEn_10330
- DNA Barcode:
- CTATTTCCCGGATACTTTTTCGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NUP210P1 (255330)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465268
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 255330 | NUP210P1 | nucleoporin 210 pseudogene 1 | NR_034158.1 | 46.5% | 1_244del;653_876del | |
| 2 | human | 23225 | NUP210 | nucleoporin 210 | XM_024453416.1 | 8.7% | 7.7% | (many diffs) |
| 3 | human | 23225 | NUP210 | nucleoporin 210 | XM_024453415.1 | 8.2% | 7.3% | (many diffs) |
| 4 | human | 23225 | NUP210 | nucleoporin 210 | XM_024453414.1 | 8.2% | 7.3% | (many diffs) |
| 5 | human | 23225 | NUP210 | nucleoporin 210 | XM_024453413.1 | 8.1% | 7.2% | (many diffs) |
| 6 | human | 23225 | NUP210 | nucleoporin 210 | XM_024453412.1 | 8.1% | 7.2% | (many diffs) |
| 7 | human | 23225 | NUP210 | nucleoporin 210 | NM_024923.4 | 4.6% | 4.1% | (many diffs) |
| 8 | human | 23225 | NUP210 | nucleoporin 210 | XM_024453411.1 | 4.5% | 4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 474
- ORF length:
- 408
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gaagggcact gtgtctgggc ttgtgcaggt ggtggatgca gagactggca 121 aggtggtcat catctctcag gacctcgttc aggtgaaggt gctgctgctt GGAGCCGTGA 181 GGATCCGCAG CCCCATCATG CAGATAAGGA TGGGCACCCA GCTGGGGAAG AATTGGATAC 241 ATCTCCCACT GCTTGAAGGA GGCAGCACAG CTTCTGGGGG CATTCCCAGT GCTGGCACCC 301 CGCTGGTCAC CAGGCCTGAC TTCTCCCCAC CGATGTCCAT CTATATCACC TGGATCACCA 361 ACCACCAGAA CCCTTTCTCC TTTGGCAATG CCATGCCAGG CCTGACCTTC CACTGGTCTG 421 TCACCAAGCG GGACATCCTG GATCTCCAAG GGCAGCATCA CAAGATGAGC CATTACCCAA 481 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 541 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 601 TATCTTGTGG AAAGGACGAC TATTTCCCGG ATACTTTTTC GAAACGCGTT AAGTCgacaa 661 tcaacctctg gattacaaaa tttgtgaaag att