Construct: ORF TRCN0000465304
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012373.1_s317c1
- Derived from:
- ccsbBroadEn_03928
- DNA Barcode:
- AATTAATGTAGTTTTCACTTTCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RRAGC (64121)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465304
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64121 | RRAGC | Ras related GTP binding C | NM_022157.4 | 100% | 100% | |
| 2 | human | 64121 | RRAGC | Ras related GTP binding C | NM_001271851.2 | 91.4% | 91.4% | 235_236ins102 |
| 3 | mouse | 54170 | Rragc | Ras-related GTP binding C | NM_017475.2 | 93.1% | 98.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1263
- ORF length:
- 1197
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc cctgcagtac ggggcggagg agacgcccct cgccggcagt tacggcgcgg 121 ccgattcgtt tccaaaggac ttcggctacg gcgtggagga ggaggaagag gaggcggcgg 181 cggcgggcgg aggggttggg gcaggggcag gcggtggctg tggtccgggg ggcgctgaca 241 gctccaagcc gaggattctg ctcatgggac tccggcgcag cggcaagtcc tccatccaga 301 aggtggtgtt tcataagatg tcacccaacg agaccctctt tttggaaagt accaacaaga 361 tttataagga tgacatttcc aatagctcct ttgtgaattt ccagatatgg gattttcctg 421 ggcaaatgga cttttttgac ccaacctttg actatgagat gatcttcagg ggaacaggag 481 cattgatata cgtcattgac gcacaggatg actacatgga ggctttaaca agacttcaca 541 ttactgtttc taaagcctac aaagttaacc cagacatgaa ttttgaggtt tttattcaca 601 aagttgatgg tctgtctgat gatcacaaaa tagaaacaca gagggacatt catcaaaggg 661 ccaatgatga ccttgcagat gctgggctag aaaaactcca tcttagcttt tatctgacta 721 gtatctatga ccattcaata tttgaagcct ttagtaaggt ggtgcagaaa ctcattccac 781 aactgccgac cttggaaaac ctattaaata tctttatatc aaattcaggt attgaaaaag 841 cttttctctt tgatgttgtc agcaaaatct acattgcaac agacagttcc cctgtggata 901 tgcaatctta tgaactttgc tgtgacatga tcgatgttgt aattgatgtg tcttGTATAT 961 ATGGGTTAAA GGAAGATGGA AGTGGAAGTG CTTATGACAA AGAATCTATG GCAATTATCA 1021 AGCTGAATAA TACAACTGTC CTTTATTTAA AGGAGGTGAC TAAATTTTTG GCACTGGTCT 1081 GCATTCTAAG GGAAGAAAGC TTTGAAAGAA AAGGTTTAAT AGACTACAAC TTCCACTGTT 1141 TCCGAAAAGC TATTCATGAG GTTTTTGAGG TGGGTGTGAC TTCTCACAGG AGCTGTGGTC 1201 ACCAGACTAG TGCCTCCAGT CTGAAAGCGC TGACACACAA TGGCACGCCA CGAAACGCCA 1261 TCTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1321 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1381 GGCTTTATAT ATCTTGTGGA AAGGACGAAA TTAATGTAGT TTTCACTTTC TCACGCGTTA 1441 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt