Construct: ORF TRCN0000465308
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004721.1_s317c1
- Derived from:
- ccsbBroadEn_04330
- DNA Barcode:
- GAGCCAATAGGTTGGGCTGGTAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCBD2 (84105)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465308
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84105 | PCBD2 | pterin-4 alpha-carbinolamin... | NM_032151.5 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 456
- ORF length:
- 390
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcggtgctc ggggcgctcg gggcgacgcg gcgcttgttG GCGGCGCTGC 121 GAGGCCAGAG CCTAGGGCTA GCGGCCATGT CATCAGGTAC TCACAGGTTG ACTGCAGAGG 181 AGAGGAACCA AGCTATACTT GACCTTAAAG CAGCAGGATG GTCGGAATTA AGTGAGAGAG 241 ATGCCATCTA CAAAGAATTC TCCTTCCACA ATTTTAATCA GGCATTTGGC TTTATGTCCC 301 GAGTTGCCCT ACAAGCAGAG AAGATGAATC ATCACCCAGA ATGGTTCAAT GTATACAACA 361 AGGTCCAGAT AACTCTCACC TCACATGACT GTGGTGAACT GACCAAAAAA GATGTGAAGC 421 TGGCCAAGTT TATTGAAAAA GCAGCTGCTT CTGTGTGCCC AACTTTCTTG TACAAAGTGG 481 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 541 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 601 AGAGCCAATA GGTTGGGCTG GTAGCACGCG TTAAGTCgac aatcaacctc tggattacaa 661 aatttgtgaa agatt