Construct: ORF TRCN0000465312
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017146.1_s317c1
- Derived from:
- ccsbBroadEn_03178
- DNA Barcode:
- ACGATTGTTGGAAAAATCTGAGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PAM16 (51025)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465312
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51025 | PAM16 | presequence translocase ass... | NM_016069.11 | 100% | 100% | |
| 2 | human | 100529144 | CORO7-PAM16 | CORO7-PAM16 readthrough | NM_001201479.2 | 11.8% | 11.8% | 1_2771delinsAT |
| 3 | mouse | 66449 | Pam16 | presequence translocase-ass... | NM_025571.1 | 88.8% | 96% | (many diffs) |
| 4 | mouse | 66449 | Pam16 | presequence translocase-ass... | XM_017317114.1 | 80.8% | 88% | (many diffs) |
| 5 | mouse | 66449 | Pam16 | presequence translocase-ass... | XM_017317113.1 | 60% | 64.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 441
- ORF length:
- 375
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc caagtacctg gcccagatca ttgtgatggg cgtgcaggtg gtgggcaggg 121 cctttgcacg ggccttgcgg caggagtttg cagccagccg ggccgcagct gatgcccgag 181 gacgcgctgg acaccggtct gcagccgctt ccaacctctc cggccTCAGC CTCCAGGAGG 241 CACAGCAGAT TCTCAACGTG TCCAAGCTGA GCCCTGAGGA GGTCCAGAAG AACTATGAAC 301 ACTTATTTAA GGTGAATGAT AAATCCGTGG GTGGCTCCTT CTACCTGCAG TCAAAGGTGG 361 TCCGCGCAAA GGAGCGCCTG GATGAGGAAC TCAAAATCCA GGCCCAGGAG GACAGAGAAA 421 AAGGGCAGAT GCCCCATACG TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 481 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 541 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAACGA TTGTTGGAAA 601 AATCTGAGGT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt