Construct: ORF TRCN0000465317
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000080.1_s317c1
- Derived from:
- ccsbBroadEn_15688
- DNA Barcode:
- TACGGATACCTTGCGCACCCCACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DGCR2 (9993)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465317
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9993 | DGCR2 | DiGeorge syndrome critical ... | NM_005137.3 | 99.9% | 99.8% | 1549C>A |
| 2 | human | 9993 | DGCR2 | DiGeorge syndrome critical ... | NM_001184781.2 | 99.3% | 99% | 328_329insGTTTCCTAG;1540C>A |
| 3 | human | 9993 | DGCR2 | DiGeorge syndrome critical ... | NM_001173533.1 | 92.4% | 92.3% | 75_76ins123;1426C>A |
| 4 | human | 9993 | DGCR2 | DiGeorge syndrome critical ... | NM_001173534.2 | 91.9% | 91.6% | 75_76ins123;205_206insGTTTCCTAG;1417C>A |
| 5 | human | 9993 | DGCR2 | DiGeorge syndrome critical ... | XR_001755406.2 | 35.9% | (many diffs) | |
| 6 | human | 9993 | DGCR2 | DiGeorge syndrome critical ... | XR_001755405.1 | 35.8% | (many diffs) | |
| 7 | human | 9993 | DGCR2 | DiGeorge syndrome critical ... | NR_033674.2 | 34.3% | (many diffs) | |
| 8 | mouse | 13356 | Dgcr2 | DiGeorge syndrome critical ... | NM_010048.3 | 87.7% | 93% | (many diffs) |
| 9 | mouse | 13356 | Dgcr2 | DiGeorge syndrome critical ... | NM_001109750.1 | 87.2% | 92.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1716
- ORF length:
- 1650
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gcccaaggca gacagcggcg ccttcctgct gctcttcctg ctcgtgctca 121 ctgtcaccga gccgctgcgg ccagagctgc ggtgcaaccc tgggcagttt gcgtgtcgca 181 gcggcaccat ccagtgcatc cccctcccct ggcagtgtga cggctgggcg acttgcgagg 241 atgagagcga cgaagccaac tgtccagaag tgaccgggga ggtgcgtcct catcatggga 301 aggaggctgt ggatccgcgg caggggcggg ccagaggagg cgacccttcg cacttccacg 361 cggtgaacgt ggcgcagccc gttcgcttca gcagtttcct agggaagtgc ccgacagggt 421 ggcaccacta cgaaggcacg gccagctgct accgggtcta cctgagcggg gagaactact 481 gggatgccgc gcagacctgc cagcgcctga atggctctct cgccaccttc tccactgacc 541 aggagctgcg ctttgtcctg gcccaggaat gggaccagcc cgagcggagc tttggttgga 601 aggaccagcg caagttgtgg gttggctatc agtatgttat cactggccgg aaccgctcct 661 tggaaggtcg ctgggaggtg gcattcaaag gctcttcaga ggtgttcctg cccccagacc 721 ccatctttgc ctcggccatg tctgagaacg acaacgtgtt ctgtgcccag cttcagtgct 781 tccatttccc caccctgcgg caccacgacc tccacagctg gcacgccgag agctgctacg 841 agaagtcttc atttctgtgt aaaagaagtc aaacatgtgt tgacatcaag gacaacgtgg 901 tggatgaagg gttctacttc acccctaagg gggacgaccc atgcctgagc tgcacctgcc 961 atggagggga gcctgagatg tgtgtggctg ctctctgtga gaggccccag ggctgccaac 1021 agtaccgcaa ggaccccaaa gagtgctgca agttcatgtg tctggaccca gatggcaaca 1081 gtctgtttga ctccatggcc agcgggatgc gcctggtcgt cagctgcatc tcctccttcc 1141 tcatcctgtc actgctgctc ttcatggtcc accggctgcg ccagcggcgc cgggagcgca 1201 tcgagtccct gattggagca aacttgcacc acttcaacct cggccgcagg atccctggct 1261 ttgattacgg cccagacggg tttggcacgg gcctcacgcc gctgcatctt tctgacgacg 1321 gagagggtgg gactttccat ttccacgacc ctccacctcc ctacacggca tacaagtacc 1381 cggacatcgg ccagcccgac gaccctccgc cgccctacga ggcctccatc cacccggaca 1441 gtgtgttcta tgaccctgca gacgatgatg cttttgagcc tgtggaggtc agcctgccag 1501 cccctgggga tggtgggagt gaaggtgcat tacTCCGGCG CCTGGAGCAG CCTCTGCCCA 1561 CTGCGGGGGC CTCTCTGGCA GACCTGGAAG ACTCTGCCGA CAGCAGCAGC GCCATGCTCG 1621 TGCCCCCTGA CCCTGCCCAG AGCGGGAGCA CCCCAGCTGC AGAGGCACTG CCAGGGGGTG 1681 GCCGCCACAG CCGCAGCTCC CTCAATACTG TGGTGTACCC AACTTTCTTG TACAAAGTGG 1741 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1801 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1861 ATACGGATAC CTTGCGCACC CCACGACGCG TTAAGTCgac aatcaacctc tggattacaa 1921 aatttgtgaa agatt