Construct: ORF TRCN0000465328
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008019.1_s317c1
- Derived from:
- ccsbBroadEn_10819
- DNA Barcode:
- CGGGGACCCTCTCATCGACTCACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FCER1G (2207)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465328
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2207 | FCER1G | Fc fragment of IgE receptor Ig | NM_004106.2 | 98.8% | 98.8% | 141_142insGTA |
| 2 | mouse | 14127 | Fcer1g | Fc receptor, IgE, high affi... | NM_010185.4 | 87.3% | 87.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 351
- ORF length:
- 261
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGATTCCAGC AGTGGTCTTG CTCTTACTCC 121 TTTTGGTTGA ACAAGCAGCG GCCCTGGGAG AGCCTCAGCT CTGCTATATC CTGGATGCCA 181 TCCTGTTTCT GTATGGAATT GTCCTCACCC TCCTCTACTG TCGACTGAAG GTAATCCAAG 241 TGCGAAAGGC AGCTATAACC AGCTATGAGA AATCAGATGG TGTTTACACG GGCCTGAGCA 301 CCAGGAACCA GGAGACTTAC GAGACTCTGA AGCATGAGAA ACCACCACAG TACCCAACTT 361 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 421 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 481 CTTGTGGAAA GGACGACGGG GACCCTCTCA TCGACTCACT ACGCGTTAAG TCgacaatca 541 acctctggat tacaaaattt gtgaaagatt