Construct: ORF TRCN0000465336
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005786.1_s317c1
- Derived from:
- ccsbBroadEn_05363
- DNA Barcode:
- ACTGCCCCGGTTCACTACGAAAGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM177A1 (283635)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465336
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 283635 | FAM177A1 | family with sequence simila... | NM_001079519.1 | 100% | 100% | |
| 2 | human | 283635 | FAM177A1 | family with sequence simila... | NM_001289022.2 | 100% | 100% | |
| 3 | human | 283635 | FAM177A1 | family with sequence simila... | NM_173607.4 | 90.2% | 90.2% | 1_69del |
| 4 | mouse | 100101807 | 1700047I17Rik2 | RIKEN cDNA 1700047I17 gene 2 | NM_001100116.1 | 81.1% | 83.3% | (many diffs) |
| 5 | mouse | 73385 | Fam177a | family with sequence simila... | NM_028527.1 | 81.1% | 83.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 705
- ORF length:
- 639
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ccaggagcca gtgggcggtg tggaacgagg agaagccgtc gcagcctcgg 121 gagctgcggc cgccgcggca ttcggggaat ctgcagggca gatgagtaac gaaagaggct 181 ttgaaaatgt agaactggga gtcataggaa aaaagaagaa agtcccaagg agagtcatcc 241 actttgttag tggtgaaaca atggaagaat atagcacaga tgaagacgaa gttgatggcc 301 tggagaagaa agatgttttg cctactgttg atccgacaaa acttacctgg ggtccctact 361 tatggtttta catgcttcgg gctgctacat caactctctc agtgtgtgac ttccttGGAG 421 AGAAGATTGC ATCTGTTTTG GGTATCAGCA CCCCAAAGTA CCAATATGCC ATTGATGAAT 481 ATTATCGGAT GAAGAAGGAG GAAGAAGAAG AAGAAGAAGA AAACAGGATG TCTGAAGAAG 541 CAGAAAAACA ATATCAACAG AATAAATTGC AGACTGATTC CATTGTTCAG ACAGATCAAC 601 CAGAGACAGT GATATCCAGC TCATTTGTGA ATGTCAATTT TGAAATGGAG GGAGACAGTG 661 AAGTAATTAT GGAAAGCAAG CAAAATCCAG TCTCTGTCCC ACCATACCCA ACTTTCTTGT 721 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 781 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 841 GAAAGGACGA ACTGCCCCGG TTCACTACGA AAGAACGCGT TAAGTCgaca atcaacctct 901 ggattacaaa atttgtgaaa gatt