Construct: ORF TRCN0000465337
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015820.1_s317c1
- Derived from:
- ccsbBroadEn_00292
- DNA Barcode:
- GTCAAATGTCTTGGATAACAATGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CENPA (1058)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465337
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1058 | CENPA | centromere protein A | NM_001809.4 | 100% | 100% | |
| 2 | human | 1058 | CENPA | centromere protein A | NM_001042426.1 | 81.4% | 81.4% | 209_210ins78 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 486
- ORF length:
- 420
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg cccgcgccgc cggagccgaa agcccgaggc cccgaggagg cgcagcccga 121 gcccgacccc gacccccggc ccctcccggc ggggcccctc cTTAGGCGCT TCCTCCCATC 181 AACACAGTCG GCGGAGACAA GGTTGGCTAA AGGAGATCCG AAAGCTTCAG AAGAGCACAC 241 ACCTCTTGAT AAGGAAGCTG CCCTTCAGCC GCCTGGCAAG AGAAATATGT GTTAAATTCA 301 CTCGTGGTGT GGACTTCAAT TGGCAAGCCC AGGCCCTATT GGCCCTACAA GAGGCAGCAG 361 AAGCATTTCT AGTTCATCTC TTTGAGGACG CCTATCTCCT CACCTTACAT GCAGGCCGAG 421 TTACTCTCTT CCCAAAGGAT GTGCAACTGG CCCGGAGGAT CCGGGGCCTT GAGGAGGGAC 481 TCGGCTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 541 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 601 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGTCAAATGT CTTGGATAAC AATGTACGCG 661 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt