Construct: ORF TRCN0000465339
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012316.1_s317c1
- Derived from:
- ccsbBroadEn_01435
- DNA Barcode:
- GCAAGTGGATTTAGGATTTTGTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPL34 (6164)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465339
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6164 | RPL34 | ribosomal protein L34 | NM_000995.4 | 100% | 100% | |
| 2 | human | 6164 | RPL34 | ribosomal protein L34 | NM_001319232.1 | 100% | 100% | |
| 3 | human | 6164 | RPL34 | ribosomal protein L34 | NM_001319234.1 | 100% | 100% | |
| 4 | human | 6164 | RPL34 | ribosomal protein L34 | NM_001319235.1 | 100% | 100% | |
| 5 | human | 6164 | RPL34 | ribosomal protein L34 | NM_001319236.1 | 100% | 100% | |
| 6 | human | 6164 | RPL34 | ribosomal protein L34 | NM_033625.3 | 100% | 100% | |
| 7 | mouse | 68436 | Rpl34 | ribosomal protein L34 | NM_001005859.3 | 91.4% | 99.1% | (many diffs) |
| 8 | mouse | 619547 | Rpl34-ps1 | ribosomal protein L34, pseu... | NM_001199350.1 | 91.4% | 99.1% | (many diffs) |
| 9 | mouse | 68436 | Rpl34 | ribosomal protein L34 | NM_001287581.1 | 91.4% | 99.1% | (many diffs) |
| 10 | mouse | 68436 | Rpl34 | ribosomal protein L34 | NM_026724.2 | 91.4% | 99.1% | (many diffs) |
| 11 | mouse | 100043876 | Gm4705 | predicted gene 4705 | XM_001481172.6 | 91.4% | 99.1% | (many diffs) |
| 12 | mouse | 100043434 | Gm4432 | predicted gene 4432 | NR_102329.1 | 31.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 417
- ORF length:
- 351
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt ccagcgtttg acataccgac gtaggctttc ctacaataca gcctctaaca 121 aaactaggct gtcccgaacc cctggtaata gaattgttta cctttatacc aagaaggttg 181 ggaaagcacC AAAATCTGCA TGTGGTGTGT GCCCAGGCAG ACTTCGAGGG GTTCGTGCTG 241 TAAGACCTAA AGTTCTTATG AGATTGTCCA AAACAAAGAA ACATGTCAGC AGGGCCTATG 301 GTGGTTCCAT GTGTGCTAAA TGTGTTCGTG ACAGGATCAA GCGTGCTTTC CTTATCGAGG 361 AGCAGAAAAT CGTTGTGAAA GTGTTGAAGG CACAAGCACA GAGTCAGAAA GCTAAATACC 421 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 481 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 541 ATATATCTTG TGGAAAGGAC GAGCAAGTGG ATTTAGGATT TTGTCTACGC GTTAAGTCga 601 caatcaacct ctggattaca aaatttgtga aagatt