Construct: ORF TRCN0000465342
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019058.3_s317c1
- Derived from:
- ccsbBroadEn_14681
- DNA Barcode:
- CTCCTTCTTTCGTAAAACACAACA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- JAK3 (3718)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465342
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3718 | JAK3 | Janus kinase 3 | NM_000215.3 | 100% | 100% | |
| 2 | human | 3718 | JAK3 | Janus kinase 3 | XM_005259896.3 | 96.3% | 96.3% | 1_129del |
| 3 | human | 3718 | JAK3 | Janus kinase 3 | XM_011527990.1 | 69.1% | 67.6% | (many diffs) |
| 4 | human | 3718 | JAK3 | Janus kinase 3 | XM_011527991.2 | 51.6% | 51.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3441
- ORF length:
- 3372
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcacctcca agtgaagaga cgcccctgat ccctcagcgt tcatgcagcc 121 tcttgtccac ggaggctggt gccctgcatg tgctgctgcc cgctcggggc cccgggcccc 181 cccagcgcct atctttctcc tttggggacc acttggctga ggacctgtgc gtgcaggctg 241 ccaaggccag cggcatcctg cctgtgtacc actccctctt tgctctggcc acggaggacc 301 tgtcctgctg gttccccccg agccacatct tctccgtgga ggatgccagc acccaagtcc 361 tgctgtacag gattcgcttt tacttcccca attggtttgg gctggagaag tgccaccgct 421 tcgggctacg caaggatttg gccagtgcta tccttgacct gccagtcctg gagcacctct 481 ttgcccagca ccgcagtgac ctggtgagtg ggcgcctccc cgtgggcctc agtctcaagg 541 agcagggtga gtgtctcagc ctggccgtgt tggacctggc ccggatggcg cgagagcagg 601 cccagcggcc gggagagctg ctgaagactg tcagctacaa ggcctgccta cccccaagcc 661 tgcgcgacct gatccagggc ctgagcttcg tgacgcggag gcgtattcgg aggacggtgc 721 gcagagccct gcgccgcgtg gccgcctgcc aggcagaccg gcactcgctc atggccaagt 781 acatcatgga cctggagcgg ctggatccag ccggggccgc cgagaccttc cacgtgggcc 841 tccctggggc ccttggtggc cacgacgggc tggggctgct ccgcgtggct ggtgacggcg 901 gcatcgcctg gacccaggga gaacaggagg tcctccagcc cttctgcgac tttccagaaa 961 tcgtagacat tagcatcaag caggccccgc gcgttggccc ggccggagag caccgcctgg 1021 tcactgttac caggacagac aaccagattt tagaggccga gttcccaggg ctgcccgagg 1081 ctctgtcgtt cgtggcgctc gtggacggct acttccggct gaccacggac tcccagcact 1141 tcttctgcaa ggaggtggca ccgccgaggc tgctggagga agtggccgag cagtgccacg 1201 gccccatcac tctggacttt gccatcaaca agctcaagac tgggggctca cgtcctggct 1261 cctatgttct ccgccgcagc ccccaggact ttgacagctt cctcctcact gtctgtgtcc 1321 agaaccccct tggtcctgat tataagggct gcctcatccg gcgcagcccc acaggaacct 1381 tccttctggt tggcctcagc cgaccccaca gcagtcttcg agagctcctg gcaacctgct 1441 gggatggggg gctgcacgta gatggggtgg cagtgaccct cacttcctgc tgtatcccca 1501 gacccaaaga aaagtccaac ctgatcgtgg tccagagagg tcacagccca cccacatcat 1561 ccttggttca gccccaatcc caataccagc tgagtcagat gacatttcac aagatccctg 1621 ctgacagcct ggagtggcat gagaacctgg gccatgggtc cttcaccaag atttaccggg 1681 gctgtcgcca tgaggtggtg gatggggagg cccgaaagac agaggtgctg ctgaaggtca 1741 tggatgccaa gcacaagaac tgcatggagt cattcctgga agcagcgagc ttgatgagcc 1801 aagtgtcgta ccggcatctc gtgctgctcc acggcgtgtg catggctgga gacagcacca 1861 tggtgcagga atttgtacac ctgggggcca tagacatgta tctgcgaaaa cgtggccacc 1921 tggtgccagc cagctggaag ctgcaggtgg tcaaacagct ggcctacgcc ctcaactatc 1981 tggaggacaa aggcctgccc catggcaatg tctctgcccg gaaggtgctc ctggctcggg 2041 agggggctga tgggagcccg cccttcatca agctgagtga ccctggggtc agccccgctg 2101 tgttaagcct ggagatgctc accgacagga tcccctgggt ggcccccgag tgtctccggg 2161 aggcgcagac acttagcttg gaagctgaca agtggggctt cggcgccacg gtctgggaag 2221 tgtttagtgg cgtcaccatg cccatcagtg ccctggatcc tgctaagaaa ctccaatttt 2281 atgaggaccg gcagcagctg ccggccccca agtggacaga gctggccctg ctgattcaac 2341 agtgcatggc ctatgagccg gtccagaggc cctccttccg agccgtcatt cgtgacctca 2401 atagcctcat ctcttcagac tatgagctcc tctcagaccc cacacctggt gccctggcac 2461 ctcgtgatgg gctgtggaat ggtgcccagc tctatgcctg ccaagacccc acgatcttcg 2521 aggagagaca cctcaagtac atctcacagc tgggcaaggg caactttggc agcgtggagc 2581 tgtgccgcta tgacccgcta ggcgacaata caggtgccct ggtggccgtg aaacagctgc 2641 agcacagcgg gccagaccag cagagggact ttcagcggga gattcagatc ctcaaagcac 2701 tgcacagtga tttcattgtc aagtatcgtg gtgtcagcta tggcccgggc cgccagagcc 2761 tgcggctggt catggagtac ctgcccagcg gctgcttgcg cgacttcctg cagcggcacc 2821 gcgcgcgcct cgatgccagc cgcctccttc tctattcctc gcagatctgc aagggcatgg 2881 agtacctggg ctcccgccgc tgcgtgcacc gcgacctggc cgcccgaaac atcctcgtgg 2941 agagcgaggc acacgtcaag atcgctgact tcggcctagc taagctgctg ccgcttgaca 3001 aagactacta cgtggtccgc gagccaggcc agagccccat tttctggtat gcccccgaat 3061 ccctctcgga caacatcttc tctcgccagt cagacgtctg gagcttcggg gtcgtcctgt 3121 acgagctctt cacctactgc gacaaaagct gcagcccctc ggccgagttc ctgcggatga 3181 tgggatgtga gcgggatgtc cccgccctct gccgcctctt ggaactgctg gaggagggcc 3241 agaggctgcc ggcgccTCCT GCCTGCCCTG CTGAGGTTCA CGAGCTCATG AAGCTGTGCT 3301 GGGCCCCTAG CCCACAGGAC CGGCCATCAT TCAGCGCCCT GGGCCCCCAG CTGGACATGC 3361 TGTGGAGCGG AAGCCGGGGG TGTGAGACTC ATGCCTTCAC TGCTCACCCA GAGGGCAAAC 3421 ACCACTCCCT GTCCTTTTCA TAGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 3481 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 3541 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACTCC TTCTTTCGTA 3601 AAACACAACA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt