Construct: ORF TRCN0000465421
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016887.1_s317c1
- Derived from:
- ccsbBroadEn_10786
- DNA Barcode:
- TATATAACATACCCTTTCGAAGCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DYNC1H1 (1778)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465421
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1778 | DYNC1H1 | dynein cytoplasmic 1 heavy ... | NM_001376.5 | 4% | 3.6% | (many diffs) |
| 2 | mouse | 13424 | Dync1h1 | dynein cytoplasmic 1 heavy ... | NM_030238.2 | 3.6% | 3.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 657
- ORF length:
- 591
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat cagtaaaatg ctgaagatgc agatgttgga ggatgaggac gacctggcct 121 acgcagagac cgagaagaag acgaggacag actccacgtc cgacgggcgc cctgcctgga 181 tgcggacact gcacaccacc gcgtccaact ggctgcacct catcccccag acgctgagcc 241 acctcaagcg caccgtggag aatatcaagg atcctttgtt caggttcttt gagagagaag 301 tgaagatggg cgcaaagctg cttcaggacg ttcgccagga ccttgcagat gtcgtccagg 361 tgtgcgaagg aaagaagaag cagaccaact acttgcgcac gctgatcaac gagctagtga 421 aagggatctt gcctcggagc tggtcccact acacggtgcc tgccggcatg accgtcatcc 481 agtgggtgtc cgacttcagc gagaggatca aacagctgca gaacaTCTCA CTGGCAGCTG 541 CATCTGGTGG CGCCAAGGAG CTAAAGGTGA AGGCGCTCCT GACGAGTCTC GGGTGGTCAG 601 CAGCTGTCCT GGGCTGGGGT GGGAGTGGCT CTGGGGAAAA ACACAGGGCC CAGGTCTGCC 661 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 721 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 781 ATATATCTTG TGGAAAGGAC GATATATAAC ATACCCTTTC GAAGCTACGC GTTAAGTCga 841 caatcaacct ctggattaca aaatttgtga aagatt