Construct: ORF TRCN0000465433
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011700.1_s317c1
- Derived from:
- ccsbBroadEn_05202
- DNA Barcode:
- TACAGATGCCATTAACTCAATACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RWDD4 (201965)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465433
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 201965 | RWDD4 | RWD domain containing 4 | NM_152682.4 | 100% | 100% | |
| 2 | human | 201965 | RWDD4 | RWD domain containing 4 | NM_001307922.2 | 66.4% | 66.4% | 0_1ins189 |
| 3 | human | 201965 | RWDD4 | RWD domain containing 4 | XR_002959716.1 | 20.3% | 1_266del;796_929del;965_2768del | |
| 4 | mouse | 192174 | Rwdd4a | RWD domain containing 4A | NM_203507.3 | 85.6% | 90.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 630
- ORF length:
- 564
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag tgccaacgag gaccaggaga tggaactaga agcattacgc tctatttatg 121 aaggagatga aagtttccgg gaattaagtc cagtttcttt tcaatatagg ataggtgaaa 181 atggtgatcc caaagccttc ttaatagaga tttcctggac agaaacatat ccccaaacac 241 ctccaattct atctatgaac gcttttttta acaacaccat atcatcagct gtaaagcaga 301 gtatattagc caagctacag gaagcagtag aagctaatct tGGAACCGCT ATGACCTATA 361 CATTGTTTGA ATATGCCAAA GACAATAAAG AGCAGTTCAT GGAGAATCAC AATCCCATCA 421 ATTCCGCAAC ATCGATAAGC AATATCATCT CAATTGAAAC TCCTAATACA GCCCCATCAA 481 GTAAGAAAAA AGACAAAAAA GAACAACTTT CAAAAGCCCA GAAGCGTAAG CTGGCAGACA 541 AAACAGATCA CAAAGGAGAA CTTCCTCGAG GCTGGAACTG GGTTGATGTT GTGAAGCATT 601 TAAGCAAAAC TGGCTCTAAG GATGATGAGT ACCCAACTTT CTTGTACAAA GTGGTTGATA 661 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 721 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATACAG 781 ATGCCATTAA CTCAATACAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 841 tgaaagatt